Isolation and characterization of mutants ofEscherichia coli defective in pyridine nucleotide cycle enzymes |
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| Authors: | Robert D. Lemmon John J. Rowe Gerald J. Tritz |
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| Affiliation: | (1) Department of Biochemistry, Purdue University, 47907 West Lafayette, Indiana, USA;(2) Department of Biology, University of Dayton, 45469 Dayton, Ohio, USA;(3) Department of Microbiology and Immunology, Kirksville College of Osteopathic Medicine, 63501 Kirksville, Missouri, USA |
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| Abstract: | Thirty-eight analogs of nicotinic acid and nicotinamide were tested for their ability to inhibit growth of wild-typeEscherichia coli K-12. Two of the compounds tested, 6-aminonicotinic acid and 6-aminonicotinamide were strongly inhibitory to growth of the organism. Mutants resistant to these compounds were isolated and characterized by cross-feeding experiments. All of the mutants isolated by their resistance to these analogs were found to excrete a metabolite which supported growth ofnadA, nadB, ornadC strains ofE. coli on a minimal medium. Wild-type strains failed to exhibit this cross-feeding ability. ThepncB + locus codes for nicotinic acid phosphoribosyl transferase and maps near minute 23 on the chromosome. |
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