Study of some physico-chemical characteristics of a Saccharomyces cerevisiae endopolygalacturonase: a possible use in beverage industry |
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| Authors: | A Belarbi C Hachet A-C Helfer S Gognies A Gainvors |
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| Affiliation: | (1) Université de Reims, Faculté des Sciences, Laboratoire de Microbiologie Générale et Moléculaire, Europol’Agro, BP 1039, 51687 Reims cedex 2, France, FR |
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| Abstract: | Zymograms of a crude protein extract from S. cerevisiae strain SCPP containing endopolygalacturonase were studied and compared to the purified enzyme by determining their physico-chemical properties. The results obtained with crude extract were similar to those of the purified enzyme. The endopolygalacturonase from both sources displayed a pH optimum between 3.0 and 4.0, and was active at temperatures between 4 and 50°C on a large panel of substrates. These characteristics make this S. cerevisiae endopolygalacturonase an attractive tool for the beverage industry. Journal of Industrial Microbiology & Biotechnology (2000) 24, 296–300. Received 24 September 1999/ Accepted in revised form 29 January 2000 |
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| Keywords: | : Saccharomyces cerevisiae pectinases polygalacturonase PGL1– 1 gene characterization |
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