温光敏核不育水稻N28S无花粉败育的显微结构观察

采用石蜡切片和荧光显微技术观察了温光敏核不育水稻N28S无花粉败育过程中的显微结构变化，结果显示：N28S的小孢子母细胞形成后细胞质变得稀薄，一部分不能进行减数分裂，一部分减数分裂阻滞在细线期或胞质分裂异常，最终所有细胞液泡化解体消失。在此过程中，还观察到小孢子母细胞在细线期胼胝质壁不产生或提早消失，以及小孢子发育后期花药壁绒毡层的异常解体。认为N28S的无花粉败育是由小孢子母细胞的细胞质异常引起的，胼胝质壁和绒毡层的异常是结果而不是原因。

Microstructure Observations of Pollenless Abortion in Thermo-and Photoperiod-sensitive Genic Male Sterile Line N28S in Rice (Oryza sativa)

Using paraffin section method and fluorescence microscopy, anther microstructure changes of pollenless abortion were observed in thenno- and photoperiod-sensitive genic male sterile line N28S in rice. The results showed that the microsporocytes of N28S had a reduced cytoplasm. Some microsporocytes didn＇t undergo meiosis, the other mierosporocytes arrested meiotic division at the leptotene stage of meiosis or underwent abnormal cytokinesis. All abnormal microsporocytes and meiotic products became vacuolate and eventually degenerated, leaving empty anther locules. Moreover, the absence or premature degradation of callose wall around the microsporocytes and the abnormal degradation of tapetum in the microspore development stage were also been observed. It is suggested that the abnormal behaviour of callose and tapetum may be a consequence of a deviation in rnicrosporocyte development, and the pollenless abortion of N28S is possibly attributed to microsporocytes with reduced cytoplasm.