Identification of a RAPD marker for palmitic-acid concentration in the seed oil of spring turnip rape (Brassica rapa ssp. oleifera) |
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| Authors: | P. K. Tanhuanpää J. P. Vilkki H. J. Vilkki |
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| Affiliation: | (1) Plant Breeding Section, Institute of Crop and Soil Science, Agricultural Research Centre of Finland, FIN-31600 Jokioinen, Finland;(2) Boreal Plant Breeding, FIN-31600 Jokioinen, Finland;(3) Animal Breeding, Institute of Animal Production, Agricultural Research Centre of Finland, FIN-31600 Jokioinen, Finland |
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| Abstract: | F2 progeny (105 individuals) from the cross Jo4002 x Sv3402 were used to identify DNA markers associated with palmitic-acid content in spring turnip rape (Brassica rapa ssp. oleifera). QTL mapping and ANOVA analysis of 140 markers exposed one linkage group with a locus controlling palmitic-acid content (LOD score 27), and one RAPD (random amplified polymorphic DNA) marker, OPB-11a, closely linked (1.4 cM) to this locus. Palmitic-acid content in the 62 F2 plants with the visible allele of marker OPB-11a was 8.45 ±3.15%, while that in the 24 plants without it was 4.59 ±0.97%. As oleic-acid concentration is affected by a locus on the same linkage group as the palmitic-acid locus, this locus probably controls the chain elongation from palmitic acid to oleic acid (through stearic acid). Marker OPB-11a may be used in future breeding programs of spring turnip rape to simplify and hasten the selection for palmitic-acid content. |
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| Keywords: | Brassica rapa RFLP RAPD QTL Palmitic acid |
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