Dog Tear Film Proteome In-Depth Analysis |
| |
| Authors: | Mateusz Winiarczyk Dagmara Winiarczyk Tomasz Banach Lukasz Adaszek Jacek Madany Jerzy Mackiewicz Dorota Pietras-Ozga Stanislaw Winiarczyk |
| |
| Affiliation: | 1. Department of Vitreoretinal Surgery, Medical University of Lublin, 20–079 Lublin, Chmielna 1, Poland.; 2. Department and Clinic of Animal Internal Diseases, University of Life Sciences, 20–612 Lublin, Głęboka 30, Poland.; 3. Department of Epizootiology and Clinic of Infectious Diseases, University of Life Sciences, 20–612 Lublin, Głęboka 30, Poland.; University of Oklahoma Health Sciences Center, UNITED STATES, |
| |
| Abstract: | In this study, mass spectrometry was used to explore the canine tear proteome. Tear samples were obtained from six healthy dogs, and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS-PAGE) was used as a first step to separate intact proteins into 17 bands. Each fraction was then trypsin digested and analysed by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS/MS) to characterize the protein components in each fraction. In total, 125 tear proteins were identified, with MCA (Major Canine Allergen), Serum albumin, UPF0557 protein C10orf119 homolog, Collagen alpha-2(I) chain, Tyrosine -protein kinase Fer, Keratine type II cytoskeletal, Beta-crystallin B2, Interleukin-6 and Desmin occuring as the most confident ones with the highest scores. The results showed that the proteomic strategy used in this study was successful in the analysis of the dog tear proteome. To the best of our knowledge, this study is the first to report the comprehensive proteome profile of tears from healthy dogs by 1D SDS PAGE and MALDI-TOF. Data are available via ProteomeXchange with identifier PXD003124. |
| |
| Keywords: | |
|
|
