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Activation loop phosphorylation tunes conformational dynamics underlying Pyk2 tyrosine kinase activation
Affiliation:1. Roy J. Carver Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA;1. Department of Chemistry, University of the Pacific, Stockton, CA 95211, USA;2. Division of Neonatology, Department of Pediatrics, The Children’s Regional Hospital at Cooper, Camden, NJ 08103, USA;3. Molecular Biophysics and Integrated Bioimaging, Berkeley Center for Structural Biology, 1 Cyclotron Road, Lawrence Berkeley Nat. Lab, Berkeley, CA 94720, USA;1. Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Université de Montréal, Québec, QC, Canada;2. Department of Anatomy and Cell Biology, Faculty of Medicine and Health Sciences, McGill University, Québec, QC, Canada;3. Montreal Heart Institute and, Université de Montréal, Québec, QC, Canada;4. Department of Nutrition, Faculty of Medicine, Université de Montréal, Québec, QC, Canada;1. Institut Pasteur, Université Paris Cité, CNRS UMR3528, Structural Bioinformatics Unit, 75015 Paris, France;2. Institut Pasteur, Université Paris Cité, CNRS UMR3528, Biochemistry of Macromolecular Interactions Unit, 75015 Paris, France;3. Institut Pasteur, Université Paris Cité, CNRS UMR3528, Biological NMR and HDX-MS Technological Platform, 75015 Paris, France;4. Institut Pasteur, Université Paris Cité, CNRS UMR3528, Crystallography Platform, 75015 Paris, France;5. Institut Pasteur, Université Paris Cité, CNRS UMR3528, Bacterial Transmembrane Systems Unit, 75015 Paris;1. Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA;2. Laboratory of Systems Pharmacology, Harvard Program in Therapeutic Science, Department of Systems Biology, Harvard Medical School, Boston, MA, USA;3. Program in Molecular and Integrative Physiological Sciences Program, Harvard T.H. Chan School of Public Health, Boston, MA, USA;4. John B. Little Center for Radiation Sciences, Harvard T.H. Chan School of Public Health, Boston, MA, USA;5. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA;6. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA;1. Center for Sickle Cell Disease, College of Medicine, Howard University, Washington, District of Columbia, USA;2. College of Dentistry, Howard University, Washington, District of Columbia, USA;3. Department of Microbiology, College of Medicine, Howard University, Washington, District of Columbia, USA;4. Department of Medicine, College of Medicine, Howard University, Washington, District of Columbia, USA;1. Department of Physiology and Biophysics, Case Western Reserve University, School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106, USA;2. Molecular Microbiology and Structural Biochemistry (MMSB, UMR 5086), CNRS & University of Lyon, 7 Passage du Vercors, 69007 Lyon, France
Abstract:
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  • Keywords:Pyk2 non-receptor tyrosine kinase  H/D exchange mass spectrometry  conformational dynamics  Src kinase  activation segment  allosteric communication
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