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Multi-site-specific isotopic labeling accelerates high-resolution structural investigations of pathogenic huntingtin exon-1
Affiliation:1. Centre de Biologie Structurale (CBS), Université de Montpellier, INSERM, CNRS, 29, rue de Navacelles, 34090 Montpellier, France;2. Institut Laue Langevin, 38000 Grenoble, France;3. LAAS-CNRS, Université de Toulouse, CNRS, 31400, Toulouse, France;4. Institut François Jacob, Molecular Imaging Center (MIRCen), Commissariat à l''Energie Atomique et aux Energies Alternatives (CEA) and Laboratory of Neurodegenerative Diseases, Centre National de la Recherche Scientifique (CNRS), Université Paris-Saclay, CEA-Fontenay-aux-Roses Bâtiment 61, 18, route du Panorama, 92265 Fontenay-aux-Rses cedex, France;1. Protein Processing Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA;2. Cancer Innovation Laboratory, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA;1. School of Biosciences, University of Sheffield, S10 2TN Sheffield, UK;1. School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China;1. Drug Discovery Biology Theme, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville 3052, VIC, Australia;2. ARC Centre for Cryo-Electron Microscopy of Membrane Proteins, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville 3052, VIC, Australia;1. Department of Physiology & Biophysics, Boston University Chobanian & Avedisian School of Medicine, Boston, MA 02118, USA;2. Department of Chemistry, The College of New Jersey, Ewing, NJ 08628, USA;3. Department of Physics, Umeå University, Umeå, Sweden;4. Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå, Sweden
Abstract:
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  • Keywords:huntingtin  Huntington’s disease  nuclear magnetic resonance  poly-Q  tRNA suppression  isotopic labeling  intrinsically disordered proteins  conformational ensemble
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