Looking into flowering time in almond (Prunus dulcis (Mill) D. A. Webb): the candidate gene approach |
| |
Authors: | C Silva J Garcia-Mas A M Sánchez P Arús M M Oliveira |
| |
Institution: | (1) Instituto de Tecnologia Química e Biológica (ITQB), Instituto de Biologia Experimental e Tecnológica (IBET), Quinta do Marquês, 2784-505 Oeiras, Portugal;(2) Departament de Genètica Vegetal, Laboratori CSIC-IRTA de Genética Molecular Vegetal, Carretera de Cabrils s/n, 08348 Cabrils-Barcelona, Spain;(3) Faculdade de Ciências, Dep. Biologia Vegetal, Universidade de Lisboa, Campo Grande, 1749-016 Lisbon, Portugal |
| |
Abstract: | Blooming time is one of the most important agronomic traits in almond. Biochemical and molecular events underlying flowering regulation must be understood before methods to stimulate late flowering can be developed. Attempts to elucidate the genetic control of this process have led to the identification of a major gene (Lb) and quantitative trait loci (QTLs) linked to observed phenotypic differences, but although this gene and these QTLs have been placed on the Prunus reference genetic map, their sequences and specific functions remain unknown. The aim of our investigation was to associate these loci with known genes using a candidate gene approach. Two almond cDNAs and eight Prunus expressed sequence tags were selected as candidate genes (CGs) since their sequences were highly identical to those of flowering regulatory genes characterized in other species. The CGs were amplified from both parental lines of the mapping population using specific primers. Sequence comparison revealed DNA polymorphisms between the parental lines, mainly of the single nucleotide type. Polymorphisms were used to develop co-dominant cleaved amplified polymorphic sequence markers or length polymorphisms based on insertion/deletion events for mapping the candidate genes on the Prunus reference map. Ten candidate genes were assigned to six linkage groups in the Prunus genome. The positions of two of these were compatible with the regions where two QTLs for blooming time were detected. One additional candidate was localized close to the position of the Evergrowing gene, which determines a non-deciduous behaviour in peach. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|