| 牙龈卟啉杆菌W83单克隆抗体的研制与鉴定 |
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| 引用本文： | 薛毅,许丽华,史俊南. 牙龈卟啉杆菌W83单克隆抗体的研制与鉴定[J]. 中国微生态学杂志, 1993, 0(2) |
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| 作者姓名： | 薛毅 许丽华 史俊南 |
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| 作者单位： | 中国人民解放军白求恩国际和平医院，河北医学院口腔医学系，第四军医大学口腔医学院 石家庄 050082 |
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| 基金项目： | 全军“八五计划”医药卫生青年基金资助 |
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| 摘 要： | 牙龈类杆菌曾是重要的产黑色素类杆菌群菌株,最近重新命名为牙龈卟啉杆菌,该菌为牙周病龈下菌斑中关键性厌氧菌,与成人慢性牙周炎的发生、发展有密切关系。作者用牙龈卟啉杆菌侵袭型菌株W83,作为免疫原,通过免疫小鼠、细胞融合、筛选、克隆化,最后得到一株能够稳定分泌抗牙龈卟啉杆菌W83的单克隆抗体杂交瘤细胞系,经鉴定该单抗特异性良好,可用于临床该菌的检出和生态学研究。
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| 关 键 词： | 牙龈卟啉杆菌 单克隆抗体 杂交瘤 |
PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODY THAT SPECIFIC FOR PORPHYROMANUS GINGIVALIS |
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| Xue Yi,Bethune International Peace Hospital of PLA. Shijiazhuang.. PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODY THAT SPECIFIC FOR PORPHYROMANUS GINGIVALIS[J]. Chinese Journal of Microecology, 1993, 0(2) |
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| Authors: | Xue Yi Bethune International Peace Hospital of PLA. Shijiazhuang. |
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| Affiliation: | Xue Yi,Bethune International Peace Hospital of PLA. 050082 Shijiazhuang. |
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| Abstract: | Porphyromanus gingivalis is known to important microorganism in the etiology of adult chronic periodontitis.In the paper, we generated a hybridoma secrecting monoclonal antibody against virulence porphyromanus gingivalis W83.The specificity of the Mc Ab was examined by ELISA and indirect immunofluorescence method against some related Bacteria.The results tell us our McAb is specific for Porphyromanus gingivalis.The McAb could be used for clinical samples detections. |
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| Keywords: | Porphyromanus gingivalis Antibody Monoclonal Hybridoma cell line |
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