| Abstract: | Genetic transformation has been studied in fragmented shootapex cultures of Vitis vinifera L. following co-cultivationwith Agrobacterium tumefaciens. Transgenic shoots of the cultivarCabernet Sauvignon, tolerant to low levels of kanamycin, havebeen produced. Proliferation of transgenic cells in the presumptivebud forming area of cultured fragments has been observed usingthe enzyme activity of ß-glucuronidase (GUS) as amarker. The distribution of GUS stained cells both in this tissueand in transgenic shoots, the presence of low copy numbers ofthe neomycin phosphotransferase II gene in transgenic shootsand the relatively low levels of kanamycin resistance suggestthat these shoots contain both transformed and untransformedcells. Key words: Grapevine, transformation, Agrobacterium, GUS, NPTII |
