Sequence studies on human placenta tRNAVal : comparison with the mouse myeloma tRNAVal

The major species of valine specific tRNA was isolated from human placenta, degraded to oligonucleotides, and shown to have the nucleotide sequence pG-U-U-U-C-C-G-U-A-G-U-G-U?-A-G-D-G-G-D-D-A-U-C-A-C-m²G-U?-U-C-G-C-C-U-(I or C)-A-C-A-C-G-C-G-A-A-A-G-m⁷G-D-m⁵C-m⁵C-C-C-G-G-U-U?-C-G-m¹A-A-A-C-C-G-G-G-C-G-G-A-A-A-C-A-C-C-A_OH. This human placental tRNA^Val differs from the major species of mouse myeloma tRNA^Val only in that it contains either I or C in the wobble position of the anticodon, and totally lacks 2′-O-methylcytosine and 5-methylcytosine in the anticodon loop.