Oil body formation in Euphorbia tirucalli L. cell suspension cultures |
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| Authors: | Kanji Ohyama Yuko Uchida Norihiko Misawa Tohru Komano Minoru Fujita Tamio Ueno |
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| Institution: | (1) Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University, 606 Kyoto, Japan;(2) Laboratory of Wood Structure, Department of Wood Science and Technology, Kyoto University, 606 Kyoto, Japan;(3) Division of Chemical Biology of Pesticides, Pesticide Research Institute, Kyoto University, 606 Kyoto, Japan |
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| Abstract: | The culture of cells of Euphorbia tirucalli L. resulted in the formation of intracellular particles which osmium tetroxide staining and electron microscopy indicated to be oil bodies. Chemical analysis (TLC, HPLC, GLC and GC-MS) disclosed steroids, triterpenoids, and diterpenoids as the major oil components of the cultured cells. |
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