| 细菌木聚糖酶高产菌的选育及产酶条件 |
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| 引用本文： | 许正宏,白云玲,孙微,陶文沂.细菌木聚糖酶高产菌的选育及产酶条件[J].微生物学报,2000,40(4):440-443. |
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| 作者姓名： | 许正宏 白云玲 孙微 陶文沂 |
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| 作者单位： | 无锡轻工大学生物工程学院无锡 214036 |
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| 基金项目： | 江苏省科委"九、五”攻关课题(BE96042) |
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| 摘 要： | 木聚糖是一种在植物体内大量存在的半纤维素,是在自然界中含量仅次于纤维素的一种可再生植物纤维。木聚糖酶(ｘｙｌａｎａｓｅ,ＥＣ3.2.1.8)是一类能够特异降解木聚糖的酶类。近年来,人们将其广泛用于造纸工业的纸浆生物处理,与其他消化酶类一起用作饲料添加剂,以及应用于食品加工工业和纺织工业等。木聚糖酶可以由许多种微生物产生1],我国多集中于霉菌木聚糖酶的研究。本文报告了一株细菌木聚糖酶产生菌的筛选及产酶条件的研究结果。1 材料和方法11 菌株本实验室分离、保存的木聚糖酶产生菌ＷＸＵＬＩ11及其突变株ＷＬＵＮ024。12 培养…
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| 关 键 词： | 木聚糖酶 诱变 发酵条件 |
| 文章编号： | 0001-6200(2000)04-0440-04 |
SCREENING AND FERMENTATION CONDITIONS OF A BACTERIAL STRAIN OVER-PRODUCING XYLANASE |
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| Xu Zhenghong\ Bai Yunling\ Sun Wei\ Tao Wenyi.SCREENING AND FERMENTATION CONDITIONS OF A BACTERIAL STRAIN OVER-PRODUCING XYLANASE[J].Acta Microbiologica Sinica,2000,40(4):440-443. |
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| Authors: | Xu Zhenghong\ Bai Yunling\ Sun Wei\ Tao Wenyi |
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| Institution: | School of Biotechnology, Wuxi University of Light Industry, Wuxi 214036. |
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| Abstract: | A xylanase producer WXULI-11 was isolated and identified as Pseudomonas sp. By combination treatment of WXULI-11 with UV and NTG, a mutant WLUN024 was obtained and its enzyme activity reached 354 IU/mL in 36 h by shaking flask. The mechanism of its xylanase production was primarily studied. The results showed that this enzyme was induced by xylan, xylose and some other xylosic material, while xylose had "two-way effect" on xylanase synthesis. Effects of nitrogen and other factors on enzyme production were also investigated. |
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| Keywords: | Xylanase Mutagensis Fermentation conditions |
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