Carboxypeptidase digestion in the presence of detergents |
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Authors: | M Kamo A Tsugita |
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Institution: | Department of Chemistry, Faculty of Science, Science University of Tokyo, Chiba. |
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Abstract: | The detergents, sodium dodecyl sulfate (SDS), lauryl glutamate (LG), and octyl-(polydisperse)oligooxyethylene (Octyl-POE), were tested as to their effects on the activities of carboxypeptidases A, B, and P. In general, Octyl-POE showed little inhibition and SDS showed the strongest inhibition. Carboxypeptidase B was only slightly inhibited by SDS. The inhibitory effect of SDS on these enzyme activities depended not on its concentration but on its absolute amount. For a constant amount of SDS, the activities of carboxypeptidases A and B remained almost constant with increasing reaction volume. Commercial carboxypeptidase B is usually contaminated by carboxypeptidase A. With the addition of SDS, almost only carboxypeptidase B activity was detected. |
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