灰树花子实体与发酵菌丝体挥发性化合物分析

分别采用固体栽培法和液体发酵法对同一灰树花菌株进行培养,获得灰树花子实体和发酵菌丝体,并采用顶空固相微萃取(HS-SPME)和气相色谱-质谱(GC-MS)分析技术对子实体和菌丝体的挥发性化合物进行了分析比较。结果表明,灰树花子实体和发酵菌丝体分别含有62种和94种挥发性物质,其中37种为相同物质,分别占总挥发性物质的86.81%和84.28%。灰树花子实体中含有醇(14种)、酮(13种)、醛(12种)、酸(7种)、酯(5种)等物质,主要以酯类(42.80%)、醛类(35.14%)物质为主,其中乙酸乙酯的相对含量达到了42.57%。发酵菌丝体中主要含有醛(22种)、酮(17种)、醇(16种)、酯(13种)、酸(12种)等挥发性物质,其中醛类、酯类、酸类、醇类分别占47.0%、10.90%、7.48%和5.94%。异戊醛和地衣酚的含量分别达到了23.31%、15.41%。

Analysis of volatile compounds in fruiting bodies and submergedly cultured mycelia of Grifola frondosa

Fruiting bodies and mycelia of Grifola frondosa were produced by solid-state and submerged cultures, respectively. The volatile compounds of fruiting bodies and mycelia were analyzed by headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS). The results showed that fruiting bodies and mycelia of Grifola frondosa, respectively, contained 62 and 94 volatile compounds. Hereinto, 37 compounds in both fruiting bodies and mycelia accounted for 86.81% and 84.28% of total volatile substances, respectively. The fruiting bodies mainly contained alcohol (14), ketones (13), formaldehyde (12), acid (7), esters (5), and so on. Esters (42.80%) and aldehydes (35.14%) were the main volatile compounds of fruiting bodies, and the relative content of ethyl acetate was 42.57%. The mycelia mainly contained formaldehyde (22), ketones (17), alcohol (16), esters (13), acid (12) and so on, of which aldehydes, esters, acids and alcohols, respectively, accounted for 47.0%, 10.90%, 7.48% and 5.94%. The content of isovaleraldehyde and lichen phenol reached 23.31% and 15.41%, respectively.