Interaction of Salivary and Midgut Proteins of <Emphasis Type="Italic">Helicoverpa armigera</Emphasis> with Soybean Trypsin Inhibitor |
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Authors: | Santosh Kumar Upadhyay Krishnappa Chandrashekar |
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Institution: | (1) National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow, 226001, UP, India; |
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Abstract: | Feeding of Helicoverpa armigera larvae on semi-synthetic diet containing Soybean trypsin inhibitor (STI) resulted in disappearance of STI sensitive protease
in salivary and midgut protease extract. This might be due to in situ inhibition by dietary STI. STI was largely degraded
within 1 h of incubation with total salivary protease (1:1). Degradation was relatively low in midgut proteases. STI interacting
proteins were isolated from saliva and midgut extracts of larvae fed on STI supplemented diet using affinity column. Most
of the isolated proteins showed caseinolytic activity in zymogram. Denovo sequencing data of seven different peptides selected
from trypsin digested total protein showed similarity to chymotrypsinogen, serine protease, aminopeptidase N, peroxidase,
hypothetical protein and muscle specific protein. |
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Keywords: | |
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