Generation and characterization of a lipopolysaccharide-specific murine monoclonal antibody to <Emphasis Type="Italic">Proteus vulgaris</Emphasis> OX19 |
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Authors: | Diprabhanu Bakshi Reena Jain Urmil Tuteja H V Batra |
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Institution: | (1) Division of Microbiology, Defence R&D Establishment, Jhansi Road, Gwalior, 474002, India;(2) Present address: Defence Food Research Laboratory, Siddarthanagar, Mysore, 570011, India |
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Abstract: | The three strains of non-pathogenic Proteus species namely, Proteus vulgaris OX2, P. vulgaris OX19 and Proteus mirabilis OXK used in the Weil–Felix test are the group-specific cross-reactive antigens for Rickettsia and Orientia species. Earlier studies have revealed that the group specific and cross-reactive antigens responsible for the Weil–Felix
test lie mostly in the lipopolysaccharide (LPS) moiety of the bacterial cell wall Amano et al. (1993a) Infect Immun 61:4350–4355, (1993b) Microbiol Immunol 37:927–933, (1998) Infect Immun 66:923–926]. The three Proteus strains (OX2, OX19 and OXK) were used to raise murine monoclonal antibodies (MAbs) by hybridoma technology. Several MAb-producing
hybridomas could be stabilized following limiting dilution. Affinity and specificity of these MAbs were checked by indirect
ELISA using a battery of homologous and heterologous antigens including LPS. Amongst these, one MAb was found to be specific
for P. vulgaris OX19 LPS. Since the Weil–Felix reaction is based on the cross-reactivity between the LPS based epitopes, this MAb could be
of potential use in mapping of epitopes on the cross-reactive LPS and may also be useful as a potential diagnostic reagent. |
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Keywords: | Lipopolysaccharide Monoclonal antibodies Sandwich ELISA Weil– Felix test |
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