L-FABP is a critical host factor for successful malaria liver stage development |
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Authors: | Mikolajczak Sebastian A Jacobs-Lorena Vanessa MacKellar Drew C Camargo Nelly Kappe Stefan H I |
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Institution: | Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109-5219, USA. |
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Abstract: | The malaria parasite liver stage produces tens of thousands of red cell-infectious forms within its host hepatocyte. It is thought that the vacuole-enclosed parasite completely depends on the host cell for successful development but the molecular parasite-host cell interactions underlying this remarkable growth have remained elusive. Using a yeast two-hybrid screen and a yeast overexpression system we show that UIS3, a parasite protein essential for liver stage development, interacts directly with liver-fatty acid binding protein, L-FABP. Down-regulation of L-FABP expression in hepatocytes severely impairs parasite growth and overexpression of L-FABP promotes growth. This is the first identified direct liver stage-host cell protein interaction, providing a possible explanation for the importance of UIS3 in liver infection. |
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Keywords: | Malaria Liver stage Hepatocyte Parasitophorous vacuole UIS3 Yeast two-hybrid Liver-fatty acid binding protein |
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