Karyotypes of three somaclonal variants and wild plants ofAllium tuberosum by bicolor FISH |
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Authors: | Geum-Sook Do Bong-Bo Seo Jae-Hong Pak In-Sun Kim Seung-Dal Song |
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Institution: | (1) Department of Biology, Kyungpook National University, 702-701 Taegu, Korea;(2) Department of Biology, Keimyung University, 704-701 Taegu, Korea |
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Abstract: | Using the fluorescence in situ hybridization (FISH) technique, we conducted karyotype analyses to identify the lost chromosomes
in three somaclonal variants obtained from tissue culture of wildAllium tuberosum (2n = 4X = 32). The three lost chromosomes of the At29 variant (2n = 29) were all chromosome 2, the two for At30 (2n = 30)
were chromosomes 7 and 8, and At31 was missing chromosome 2. Chromosome compositions of these variants were confirmed as being
fixed lines during two years of greenhouse cultivation. The bicolor FISH technique, involving both 5S and 18S–5.8S–26S ribosomal
RNA genes as probes, was used to assign chromosomal locations and to confirm whether the lost chromosomes contained any rRNA
markers. The 5S rRNA gene signals in all variants as well as the wild type were detected as two sets, one on the intercalary
region of the short arm of chromosome 3, the other on the intercalary region of the long arm of chromosome 6. One 18S–5.8S–26S
rRNA gene site on the secondary constriction included a flanking satellite and terminal region on the short arm of chromosome
8. Signals of the 18S–5.8S–26S rRNA gene in At30 showpd in only three chromosomes, indicating that one of the lost chromosomes
was chromosome 8. Overall, three marker chromosomes were established by FISH, using rRNA multigene families. |
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Keywords: | Allium tuberosum chromosomal location FISH rRNA gene |
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