Chromosomal assignment of a large tRNA gene cluster (tRNALeu,tRNAGln, tRNALys,tRNAArg, tRNAGly) to 17p13.1 |
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Authors: | N Morrison J P Goddard D H Ledbetter E Boyd D Bourn J M Connor |
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Institution: | (1) The University Department of Medical Genetics, Duncan Guthrie Institute, G3 8SJ Yorkhill, Glasgow, UK;(2) Department of Biochemistry, University of Glasgow, G12 8QQ, Glasgow, UK;(3) Institute for Molecular Genetics, Baylor College of Medicine, 77030 Houston, TX, USA |
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Abstract: | Summary A cluster of tRNA genes (tRNA
UAG
Leu
, tRNA
CUG
Gln
, tRNA
UUU
Lys
, tRNA
UCU
Arg
) and an adjacent tRNA
GCC
Gly
have been assigned to human chromosome 17p12–p13.1 by in situ hybridization using a 4.2 kb human DNA fragment for tRNALeu, tRNAGln, tRNALys, tRNAArg, and, for tRNAGly, 1.3 kb and 0.58 kb human DNA fragments containing these genes as probes. This localization was confirmed and refined to 17p13.100–p13.105 using a somatic cell hybrid mapping panel. Preliminary experiments with the biotiny lated tRNA Leu, Gln, Lys, Arg probe and metaphase spreads from other great apes suggest the presence of a hybridization site on the long arm of gorilla (Gorilla gorilla) chromosome 19 and the short arm of orangutan (Pongo pygmaeus) chromosome 19 providing further support for homology between HSA17, GGO19 and PPY19. |
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Keywords: | |
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