BRCA-1 Gene Expression and Comparative Proteomic Profile of Primordial Follicles from Young and Adult Buffalo (Bubalus bubalis) Ovaries |
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| Authors: | Vijayakumar Govindaraj Harshini Krishnagiri Manmohan Singh Chauhan |
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| Affiliation: | 1. Department of Biochemistry, Indian Institute of Science, Bangalore, India;2. Embryo Biotechnology Lab, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, India |
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| Abstract: | In our previous study, we demonstrated that the repair efficiency of DNA double-strand breaks declines with increasing age in rat primordial follicles. In the present study, we extended our studies to buffalo (Bubalus bubalis) wherein we studied the expression of BRCA-1 related DNA repair genes in primordial follicles of young (12 months-22 months) and adult (72–96 months) buffaloes. The relative expression of selected genes, as determined by RT-PCR, revealed a significant (p?
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| Keywords: | BRCA1 Bubalus bubalis DNA repair primordial follicles proteomics water buffalo |
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