Identification of Four SNPs in LHB Gene and Their Associations with Sperm Qualities of Chinese Buffaloes |
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| Authors: | Yunyun Cheng Jiaming Gu Han Xue Qiang Li Mingming Liang Nan Wang |
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| Institution: | 1. College of Animal Science, Jilin University, Changchun, China;2. China Institute of Veterinary Drug Control, Beijing, China |
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| Abstract: | Luteinizing hormone beta polypeptide (LHB) gene has been considered important for sexual behavior and has associations with sperm quality. In this study, four SNPs (g.276?T>C, g.377A>C, g.401T>C, and g.412A>G) were detected in the LHB gene of 165 water buffaloes by direct sequencing and identification of overlap peaks, each of which was associated with at least one sperm quality trait of ejaculate volume, sperm concentration, post-thaw sperm motilities, and sperm abnormalities by chi-square analysis. Among them, g.276?T>C was associated with ejaculate volume (F?=?2.857, p?C was related to ejaculate volume (F?=?4.178, p?C had a marker effect on sperm abnormalities (F?=?3.332, p?G was associated with sperm concentration (F?=?3.579, p?LHB gene and Chinese water buffalo sperm quality traits. To the best of our knowledge, this is the first report addressing the associations between the SNPs in the LHB gene and the sperm qualities of Chinese buffaloes. |
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| Keywords: | Chinese water buffalo LHB gene single-nucleotide polymorphisms (SNPs) sperm quality |
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