The bacterial Tn<Emphasis Type="Italic">9</Emphasis> chloramphenicol resistance gene: an attractive DNA segment for <Emphasis Type="Italic">Mos1 mariner</Emphasis> insertions |
| |
Authors: | Gwénaëlle Crénès Dina Ivo Joan Hérisson Sarah Dion Sylvaine Renault Yves Bigot Agnès Petit |
| |
Institution: | 1.GICC, UMR CNRS 6239, Université Fran?ois Rabelais de Tours, UFR des Sciences et Techniques,Tours,France;2.CNRS, UMR 6239, UFR des Sciences et Techniques,Tours,France;3.CHRU de Tours, UFR des Sciences et Techniques,Tours,France;4.GENNETEC, Programme d’épigénomique, Genopole,Evry Cedex,France |
| |
Abstract: | The eukaryotic mariner transposons are currently thought to have no sequence specificity for integration other than to insert within a TA contained
in a degenerated TA]1–4 tract, either in vitro or in vivo. We have investigated the properties of a suspected hotspot for the integration of the
mariner Mos1 element, namely the Tn9 cat gene that encodes a chloramphenicol acetyl transferase. Using in vitro and bacterial transposition assays, we confirmed that
the cat gene is a preferential target for MOS1 integration, whatever its sequence environment, copy number or chromosomal locus.
We also observed that its presence increases transposition rates both in vitro and in bacterial assays. The structural and
sequence features that constitute the attractiveness of cat were also investigated. We first demonstrated that supercoiling is essential for the cat gene to be a hot spot. In contrast to the situation for Tc1-like elements, DNA curvature and bendability were not found to affect integration target preferences. We found that Mos1 integrations do not occur randomly along the cat gene. All TA dinucleotides that are preferred for integration were found within either TATA or TA×TA motifs. However, these
motifs are not sufficient to constitute an attractive dinucleotide, since four TATA and TA×TA sites are cold spots.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
| |
Keywords: | Transposon Integration Target |
本文献已被 SpringerLink 等数据库收录! |
|