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Effects of antioxidants on the plant regeneration and GUS expressive frequency of peanut (<Emphasis Type="Italic">Arachis hypogaea</Emphasis>) explants by <Emphasis Type="Italic">Agrobacterium tumefaciens</Emphasis>
Authors:Email author" target="_blank">Zheng?QiushengEmail author  Ju?Bao  Liang?Likun  Xiao?Xianhua
Institution:(1) Ocean School, Yantai University, Yantai, Peoplersquos Republic of China
Abstract:Our objective is to develop an Agrobacterium-based transformation system for peanut. Ascorbic acid (AA), sodium selenite (Se), DL-agr-tocopherol (TOC) and glutathione (GSH) were used as antioxidants during the plant regeneration and co-cultivation with Agrobacterium tumefaciens. Percentage of explants with buds or shoots increased from 50 in control group to 88, 90, 87 and 76 in GSH, TOC, Se or AA treated groups, respectively. The percentage of GUS-positive plantlets increased from 3.9 (in control) to 14.5, 10.3, 12.4 and 3.9 in GSH, TOC, Se or AA groups, respectively. Some of the callus in AA group became brown and died 2 months later. GSH, TOC and Se not only eliminated the formation of H2O2 produced in wound tissue during preparation of leaflets and co-cultivation with Agrobacterium tumefaciens and decreased malondialdehyde (MDA) formation, but also enhanced superoxide dismutase (SOD) and catalase (CAT) activities. As a result, GSH, TOC or Se increased the frequency of plant regeneration and transformation efficiency of peanut (Arachis hypogaea L.) explants by Agrobacterium tumefaciens. AA is an unsuitable antioxidant in Murashige and Skoog (MS) medium due to the stimulation of oxidation in the presence of iron in MS medium.
Keywords:Arachis hypogaea L    ascorbic acid  catalase  genetic transformation  glutathione  GUS gene  hydrogen peroxide  malondialdehyde  sodium selenite  superoxide  DL-DL-agr-tocopherol" target="_blank">gif" alt="agr" align="BASELINE" BORDER="0">-tocopherol
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