Production of antibodies specific to human chorionic gonadotropin in mice immunized against its chemical analogs |
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Authors: | Kambadur Muralidhar Om P Bhal |
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Institution: | (1) School of Life Sciences, University of Hyderabad, 500 134 Hyderabad;(2) Department of Biological Sciences, Division of Cell and Molecular Biology, S.U.N.Y. at Buffalo, 14260 Buffalo, N.Y., USA |
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Abstract: | Mice immunized against DS5-hCG-Β and DS6-hCG-Β, chemical analogs of Β-subunit of human choriogonadotropin (hCG-Β) in which 5 and 6 disulphide bonds respectively were
reduced and alkylated, were found to produce antibodies specific to hCG without significant crossreactivity with human lutropin
(hLH) as tested in a radioimmunoassay. In contrast, mice immunized against the native hCG-Β subunit produced hLH crossreacting
antibodies. While the anti-DS5, DS6-hCG-Β serum was capable of selectively blocking the binding of 125I]-hCG to rat testicular LH/hCG receptors, it failed to inhibit the binding of 125I]-hLH to the same receptors. The radioimmunoassay for hCG using the mouse anti-DS5, DS6-hCG-Β serum was not as sensitive as that employing rabbit anti-DS5, DS6-hCG-Β serum. The minimal detection limit was 5 ng/ml for the mouse antibody as compared to 1 ng/ml for the rabbit antibody.
Supported by U.S. Public Health Service Grant HD 08766 to OPB.
An erratum to this article is available at . |
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Keywords: | hcG Specific antibody disulphide modified hCG-Β -subunit immunocontraceptive |
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