The isolation and characterization of gibberellin-deficient mutants in tomato |
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Authors: | M Koornneef T D G Bosma C J Hanhart J H van der Veen J A D Zeevaart |
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Institution: | (1) Department of Genetics, Agricultural University, Dreijenlaan 2, NL-6703 HA Wageningen, The Netherlands;(2) MSU-DOE Plant Research Laboratory, Michigan State University, 48824-1312 East Lansing, MI, USA |
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Abstract: | Summary In tomato, nine independent EMS-induced mutants representing recessive mutations at three different loci (gib-1, gib-2, and gib-3) were isolated. Six of these have an almost absolute gibberellin requirement for seed germination and elongation growth. In addition, the leaves are darker green, smaller, and changed in structure as compared to wild type. The three other mutants, which germinate without GA, are allelic to specific, nongerminating mutants and have less severe mutant characteristics. The respective loci are situated on three different chromosomes. The genes identified by these mutants control steps in gibberellin biosynthesis, as endogenous gibberellins are strongly reduced. |
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Keywords: | Lycopersicon esculentum Gibberellin Germination Dwarf mutants Gene localization |
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