Egr-1基因与肿瘤腺癌放射敏感性关系的研究

目的：研究Egr-1基因在2种腺癌细胞A549和Hela放射前后的表达变化及对放射敏感性的影响。方法：培养肺腺癌A549细胞和宫颈腺癌Hela细胞，分别提取4Gyx射线照射前及照射后不同时间点的细胞的总RNA行荧光定量PCR（FQ-PCR）检测Egr-1表达水平；收获4GyX射线照射前及照射后不同时间点的细胞处理行流式细胞术检测其凋亡；对照射不同剂量的细胞继续培养10-14天，进行克隆形成计数，计算克隆形成率及存活分数。结果：FQ-PCR结果显示，放射后Egr．1基因表达水平在2种细胞中均明显升高且于放射后1h达到峰值，A549细胞的峰值明显高于Hela细胞；流式细胞术检测结果显示，A549细胞凋亡明显高于Hela细胞；克隆形成实验结果显示，A549细胞存活分数明显低于Hela细胞。结论：Egr-1基因在不同腺癌细胞表达水平不同并影响其放射敏感性。

Research of the Relationship between Egr-1 and Radiosensibility of Adenoearcinoma

Objective： To research the different expression of Egr-1 in A549 and Hela cell before and after Irradiation, and the ef- fect it has on radiosensibility of Adenocarcinoma. Methods： First, culture cell adenocarcinoma of lung （A549） and adenocarcinoma of cervix （Hela）. Second, extract RNA of all cells before and after Irradiation of 4 Gy X-Ray and detect the expression level of Egr-1 using Fluorescence quantitative PCR （FQ - PCR）; Third, collect and process the cells at different times before and after 4 Gy X-ray Irradiation, and detect their apoptosis level by flow cytometry （FCM）. Forth, culture cells Irradiated by various doses for added 10 to 14 days continu- ously, and then, do clone forming assay to count colony-forming efficiency （CFE） and surviving fraction. Results： The result of FQ-PCR shows that expression levels of Egr-1 all evidently increase in the two cells after Irradiation, and reach their peak on 1 h after Irradiation; and the expression level of Egr-1 in A549 is significant higher than that in Hela cell. The result of FCM shows that the apoptosis level of A549 is much higher than that of Hela cell. The result of clone forming assay shows that the surviving fraction ofA549 is significant lower than that of Hela cell. Conclusions： The Egr-1 has different expression levels in different adenocarcinoma cell and affects the radiosensi- bility.