Somatic embryogenesis in mature zygotic embryo culture of Glehnia littoralis |
| |
Authors: | Hirai Goh Kasai Noboru Harada Takashi |
| |
Affiliation: | (1) Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060, Japan |
| |
Abstract: | In vitro somatic embryogenesis of Glehnia littoralis Fr. schm. was observed when zygotic embryos were cultured on a medium containing 1-naphthaleneacetic acid or 2,4-dichlorophenoxyacetic acid (0.01-10 μM), with 1 μM being the optimum. Microscopic observations revealed globular, heart-shaped and torpedo-shaped embryo formations and plantlet regeneration. These somatic embryos seemed to be produced directly from cells of the zygotic embryos used as explants. Of seven types of media tested, Nitsch's medium showed the highest rate of somatic embryogenesis. Somatic embryos developed into normal plantlets and were able to be potted. This revised version was published online in June 2006 with corrections to the Cover Date. |
| |
Keywords: | in vitro propagation medicinal plants tissue culture |
本文献已被 SpringerLink 等数据库收录! |
|