prp4 from Schizosaccharomyces pombe, a mutant deficient in pre-mRNA splicing isolated using genes containing artificial introns |
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Authors: | George H. Rosenberg, Suresh K. Alahari Norbert F. Kä ufer |
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Affiliation: | (1) Department of Bioscience and Biotechnology, Drexel University, 19104 Philadelphia, PA, USA |
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Abstract: | Summary We have generated a bank of temperature-sensitive (ts) Schizosaccharomyces pombe mutant strains. About 150 of these mutants were transformed with a ura4 gene containing an artificial intron. We screened these is mutants for mutants deficient in splicing of the ura4 intron. With this approach three mutants were isolated which have a general defect in the splicing process. Two of these mutants fall into the prp1 complementation group and one defines a new complementation group, prp4. |
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Keywords: | Schizosaccharomyces pombe Fission yeast Temperature-sensitive mutants Splicing Artificial introns |
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