Effects of GP2 expression on secretion and endocytosis in pancreatic AR4-2J cells |
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Authors: | Yu Su Hao Ying Lowe Anson W |
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Institution: | Department of Medicine and the Digestive Disease Center, Stanford University, Stanford, CA, USA. |
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Abstract: | GP2 is the major membrane protein present in secretory granules of the exocrine pancreas. GP2's function is unknown, but a role in digestive enzyme packaging or secretion from secretory granules has been proposed. In addition, GP2 has been proposed to influence endocytosis and membrane recycling following stimulated secretion. Adenovirus-mediated GP2 overexpression in the rat pancreatic cell line AR4-2J was used to study its impact on digestive enzyme secretion and membrane recycling. Immunoelectron microscopy showed that GP2 and amylase co-localized in secretory granules in infected AR4-2J cells. CCK-8 stimulation resulted in a fourfold increase in amylase secretion with or without GP2 expression. GP2 expression also did not influence endocytosis following CCK-8 stimulation. Thus, GP2 expression in AR4-2J cells does not affect amylase packaging in secretory granules or stimulated secretion. GP2 expression also does not influence membrane recycling in response to stimulated stimulation in AR4-2J cells. |
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Keywords: | Pancreas Exocrine Membrane protein Secretion Secretory granule Secretory pathway Endocytosis GP2 |
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