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| 野生罂粟COR基因克隆及转化 | |
| 引用本文： | 梁倩倩,魏玉杰,张金文,陈志国,贾小霞,何庆祥.野生罂粟COR基因克隆及转化[J].西北植物学报,2009,29(8). |
| 作者姓名： | 梁倩倩 魏玉杰 张金文 陈志国 贾小霞 何庆祥 |
| 作者单位： | 1. 河西学院,甘肃张掖,734000甘肃农业大学,农学院,兰州,730070 2. 甘肃农垦农业研究院,甘肃武威,733006 3. 甘肃农业大学,农学院,兰州,730070 |
| 基金项目： | 教育部博士学科点研究基金，甘肃省卫生厅重点中医药科技项目，甘肃省卫生厅重点中医药科技项目 |
| 摘 要： | 以野生罂粟幼叶总RNA为模板,采用RT-PCR技术克隆到可待因酮还原酶基因COR的cDNA序列,所获得的cDNA序列全长966 bp,具有完整的ORF,编码321个氨基酸.Blast分析表明,该片段与GenBank中的可待因酮还原酶基因(COR)家族相似性很高,其中与基因COR1.1的一致性最高可达98.96%,该片段命名为COR(GenBank,登录号为FJ624147).以中间载体pHANNIBAL和植物表达载体pART27为基础,构建了CaMV-35S启动子驱动的含可待因酮还原酶基因片段反向重复序列的RNAi双元表达载体pARC,转化烟草获得转基因植株. |
| 关 键 词： | 可待因酮还原酶 RNAi载体构建 转化 |
Cloning and Transformation of COR Gene of Papaver nudicaule |
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| LIANG Qian-qian,WEI Yu-jie,ZHANG Jin-wen,CHEN Zhi-guo,JIA Xiao-xia,HE Qing-xiang.Cloning and Transformation of COR Gene of Papaver nudicaule[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(8). | |
| Authors: | LIANG Qian-qian WEI Yu-jie ZHANG Jin-wen CHEN Zhi-guo JIA Xiao-xia HE Qing-xiang |
| Institution: | LIANG Qian-qian1,2,WEI Yu-jie3,ZHANG Jin-wen2,CHEN Zhi-guo3,JIA Xiao-xia2,HE Qing-xiang3(1 Hexi University,Zhangye,Gansu 734000,China,2 College of Agronomy,Gansu Agricultural University,Lanzhou 730070,3 Gansu Academy of Reclamation Agricultural Research,Wuwei,Gansu 733006,China) |
| Abstract: | The codeinone reductase gene was cloned from young leaf of Papaver nudicaule by RT-PCR and the coding sequences of this gene were analyzed.The result indicated that the full-length cDNA is 966 bp and contain open reading frame(ORF) encoding 321 amino acids.The sequences were analyzed with software Blast and found highly homologous with the COR gene family members and exhibited homologous of 98.96% with COR1.1.Then the promoter CaMV 35S driven,plant siRNA expression vector pARC with inverted repeats of COR w... |
| Keywords: | codeinone reductase gene construction of RNAi plant expression vector transformation |
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