Identification of native Escherichia coli BL21 (DE3) proteins that bind to immobilized metal affinity chromatography under high imidazole conditions and use of 2D-DIGE to evaluate contamination pools with respect to recombinant protein expression level |
| |
Authors: | Bartlow Patrick Uechi Guy T Cardamone John J Sultana Tamanna Fruchtl McKinzie Beitle Robert R Ataai Mohammad M |
| |
Institution: | Department of Bioengineering, 306 Center for Biotechnology, 300 Technology Drive, University of Pittsburgh, Pittsburgh, PA 15219, USA. |
| |
Abstract: | Immobilized metal affinity chromatography (IMAC) is a widely used purification tool for the production of active, soluble recombinant proteins. Escherichia coli proteins that routinely contaminate IMAC purifications have been characterized to date. The work presented here narrows that focus to the most problematic host proteins, those retaining nickel affinity under elevated imidazole conditions, using a single bind-and-elute step. Two-dimensional difference gel electrophoresis, a favored technique for resolving complex protein mixtures and evaluating their expression, here discerns variation in the soluble extract pools that are loaded in IMAC and the remaining contaminants with respect to varied levels of recombinant protein expression. Peptidyl-prolyl isomerase SlyD and catabolite activator protein (CAP) are here shown to be the most persistent contaminants and have greater prevalence at low target protein expression. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|