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Promoter knock-in: a novel rational method for the fine tuning of genes |
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| Authors: | Marjan De Mey Jo Maertens Sarah Boogmans Wim K Soetaert Erick J Vandamme Raymond Cunin Maria R Foulquié-Moreno |
| Affiliation: | (1) Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Centre of Expertise - Industrial Biotechnology and Biocatalysis, Coupure links 653, B-9000 Ghent, Belgium;(2) Department of Applied Mathematics, Biometrics and Process Control, Faculty of Bioscience Engineering, Ghent University, BIOMATH, Coupure links 653, B-9000 Ghent, Belgium;(3) Laboratory for Genetics and Microbiology, Department of Applied Biological Sciences, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium;(4) Laboratory of Molecular Cell Biology, Katholieke Universiteit Leuven, Belgium;(5) Department of Molecular Microbiology, Flanders Institute for Biotechnology, Kasteelpark Arenberg 31, B-3001 Leuven-Heverlee, Belgium |
| Abstract: | BackgroundMetabolic engineering aims at channeling the metabolic fluxes towards a desired compound. An important strategy to achieve this is the modification of the expression level of specific genes. Several methods for the modification or the replacement of promoters have been proposed, but most of them involve time-consuming screening steps. We describe here a novel optimized method for the insertion of constitutive promoters (referred to as "promoter knock-in") whose strength can be compared with the native promoter by applying a promoter strength predictive (PSP) model. |
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