Plant regeneration from cell suspension cultures of Vigna aconitifolia |
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Authors: | A S Kumar O L Gamborg M W Nabors |
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Institution: | (1) Tissue Culture for Crops Project, Department of Biology, Botany section, Colorado State University, 80523 Fort Collins, CO, USA |
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Abstract: | Plant regeneration has been achieved routinely from established cell suspension culture lines of Vigna aconitifolia (moth bean), a highly drought tolerant grain legume. The cultures originated from three-week-old leaf callus. Several media including MS, B5, AA, SL, PCM, SH and L-6 were tested for their effects on cell growth. Maximum growth was observed in L-6 medium containing 44.5 M 2,4-D. After 6 to 8 weeks the suspensions were filtered through 500, 250, 125 and 60 m sieves, respectively, for four to five subcultures. An embryogenic cell line (VA-686) was obtained from the cell fraction collected below 250 m. The VA-686 cell line is being maintained on L-6 medium with 4.5 M 2,4-D and 2.3 M Zeatin. Somatic embryogenesis was induced by transferring the cells to L-6 medium with 4.6 M zeatin in which green cell clusters were produced. The somatic embryos developed from most of the cell clusters when plated on L-6 agar medium with 2.3 M BA.Plantlets were obtained from the embryos on L-6 medium with 10.0 M IBA. The regenerated plants were grown to maturity in the greenhouse.Abbreviations BA
Benzyladenine
- 2,4-D
2,4- dichlorophenoxyacetic acid
- GA3
Gibberellic acid
- IBA
Indole-3-butyric acid
- IPA
Isopentenyladenine
- KN
Kinetin
- NAA
Napthaleneacetic acid
- AA
Toriyama and Hinata, 1985
- SL
Phillips and Collin, 1980
A project sponsored by United States Agency for International Development, Washington D.C. |
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