应用AFLP标记对江西省猕猴桃种质资源的鉴别及其分类意义

 对江西省猕猴桃种质资源进行扩增片段长度多态性(AFLP)标记来鉴定分析.首先从64对引物筛选出4对引物,对31份种质材料的DNA进行检测，得到190个扩增基因位点，其中多态性位点179个，多态性比例为94.2%，对31份种质材料的区分率达到100%.然后,对扩增结果进行UPGMA聚类分析，谱系图显示,31份种质材料之间的相似系数在0.50～0.85之间，表明猕猴桃种质之间遗传关系相对来说不是很近.在相似系数0.56的水平上，可以将31份种质大致分为4个类群：净果组和斑果组为一类群；糙毛组为一类群；星毛组为一类群；中华猕猴桃和美味猕猴桃为一类群.从树状图中看，原为中华猕猴桃的一个变种的“赣猕5号”，现与中华猕猴桃并列，有对其作进一步分类方面深入研究的必要.本研究从分子角度鉴定分析了江西省猕猴桃种质资源及其遗传关系，其结果在很大程度上与传统分类是一致的，同时也为江西省猕猴桃种质资源分类学研究提供了新的证据.

Identification of the Kiwifruit Germplasms in Jiangxi Province by AFLP and Its Classification Significance

The kiwifruit germplasms in Jiangxi province were identified by amplified fragment length polymorphism(AFLP)markers．Four primer pairs that had been selected from 64 ones had detected a total of 190 bands among 31 germplasms of kiwifruit,one hundred and seventy nine bands that representing 94．2％of total bands were polymorphic．The identification rates of 31 germplasms were up to 100％．The result suggests that applying AFLP markers to analyze the kiwifruit germplasms is feasible．Then Clustering analysis the AFLP resuit by UPGMA．The dendrogram indicated that the Dice similarity coefficients of 31 germplasms of kiwifruit ranged from 0．50 to 0．85,it suggested that their genetic relationships weren't near．Thirty one germplasms could divide into four groups according to the Dice similarity coefficient 0．56． Sect．Leiocarpae and Sect．Maculatae clustered into one group；A．Melliana of Sect．Strigosae was one group；A．Chinensis var. Rufopulpa,A．Eriantha,A．Fulvicoma var．Lenata,A．Styracifolia and A．Jiangxiensis in Sect．Stellatae clustered into the same group；but the two germplasms of Sect．Stellatae,A．Deliciosa and A．Chinensis clustered into the other group．Interestingly,the‘Ganmi-5’was in the same rank with A．Chinensis from the dendrogram,but it was the variety of A．Chinensis according to the traditional classification．It is necessary that the‘Ganmi-5’should be further researched in classification．The genetic relationships among the kiwifruit germplasms in Jiangxi province were identified and characterized by the molecular method,which was consistent with the traditional classification in certain degree and provided new evidences for the classification of the kiwifruit germplasms．