A strategy for production of monoclonal antibodies to Echinococcus granulosus antigen 5 and antigen B. |
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| Authors: | D Liu M D Rickard M W Lightowlers |
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| Affiliation: | University of Melbourne, Veterinary Clinical Centre, Werribee, Victoria, Australia. |
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| Abstract: | Serum antibody responses to sheep hydatid cyst fluid (SHCF) and a purified Antigen 5 (Ag5) were examined in ELISA, immunoelectrophoresis (IEP) and immunoprecipitation (IP) to facilitate production of monoclonal antibodies (MAb) to E. granulosus Ag5 and Antigen B (AgB). Although sera from mice immunized with SHCF contained antibodies of various classes, the fusions using these donor mice resulted in mainly anti-AgB MAb, possibly due to the preferential selection of MAb to AgB by the SHCF-based ELISA screening system. Donor mice immunized with Ag5 also produced several classes of antibodies, and the resultant fusions enabled selection of IgG MAb to Ag5. |
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