Gap junctions and zonulae occludentes of hepatocytes during biliary atresia in the lamprey |
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| Authors: | J H Youson L C Ellis D Ogilvie R R Shivers |
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| Affiliation: | 1. Department of Zoology, University of Toronto, Toronto, Ontario, Canada;2. Department of Anatomy, University of Toronto, Toronto, Ontario, Canada;3. Scarborough Campus, University of Toronto, Toronto, Ontario, Canada;4. Department of Zoology, University of Western Ontario, London, Ontario, Canada;1. Legal Medicine Unit, IRCCS Fondazione Istituto Neurologico Nazionale C. Mondino, Pavia, Italy;2. Unit of Legal Medicine and Forensic Science, Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Pavia, Italy;3. Unit of Biostatistics and Clinical Epidemiology, Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Pavia, Italy;1. Graduate School of Science and Technology, Shizuoka University, 836 Oya, Suruga-ku, Shizuoka City, Shizuoka, 422-8529, Japan;2. Department of Biology, Faculty of Science, Shizuoka University, 836 Oya, Suruga-ku, Shizuoka City, Shizuoka, 422-8529, Japan;3. Laboratory of Molecular Biology, Faculty of Pharmacy, Osaka Ohtani University, 3-11-1 Nishikiorikita, Tondabayashi City, Osaka, 584-8540, Japan;1. Graduate School of Science and Technology, Shizuoka University, Shizuoka, Japan;2. Department of Biology, Faculty of Science, Shizuoka University, Shizuoka, Japan;3. Department of Science Education, Faculty of Education, Shizuoka University, Shizuoka, Japan;4. Cetacean Resources Group, Oceanic Resources Division, National Research Institute of Far Seas Fisheries, Japan Fisheries Research and Education Agency, Yokohama, Japan |
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| Abstract: | Gap junctions and zonulae occludentes of hepatocytes were examined in thin sections and freeze-fracture replicas from livers of larval and juvenile adult lampreys and during the phase of metamorphosis when bile ducts and bile canaliculi disappear (biliary atresia). Larvae possess zonulae occludentes at the canaliculi which are composed of one to five (mean = 2.81) junctional strands that provide a bile-blood barrier. Morphometry demonstrates that during biliary atresia the decreases in number of junctional strands and apico-basal depth of the zonulae occludentes are accompanied by an increase in the frequency of gaps or interruptions in the strands and in a breakdown of the bile-blood barrier. The zonulae occludentes completely disappear during metamorphosis and are not found in the adult liver. Gap junctions of the larval liver occupy 1% of the surface of the plasma membrane and have a mean area of 0.167 micron 2 but, following an initial decline in these parameters during early biliary atresia, they rise sharply in later stages of metamorphosis and in adults are 3.2% and 0.502 micron 2, respectively. The events of alteration in junctional morphology during lamprey biliary atresia is in many ways comparable to the changes in gap junctions and zonulae occludentes during experimental and pathological intra- and extrahepatic cholestasis in mammals. |
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