| 洋紫荆凝集素的分离纯化和性质 |
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| 引用本文： | 彭建宗,陈兆平,程双奇. 洋紫荆凝集素的分离纯化和性质[J]. 植物学通报, 2000, 17(3): 266-269 |
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| 作者姓名： | 彭建宗 陈兆平 程双奇 |
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| 作者单位： | 华南师范大学生物系!广州510631 |
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| 摘 要： | 利用酸化处理的Ｓｅｐｈａｒｏｓｅ ６Ｂ亲和柱从洋紫荆种子中分离纯化出了洋紫荆凝集素（ＢＶＬ），其比活性比抽提液提高了１５９倍，活力回收率为４９．０％。ＢＶＬ分子量为８１０００，由两个相同的亚基组成。等电聚焦凝胶电注测得其等电点为４．９５。其紫外吸收高峰在２７６ｎｍ处。ＢＶＬ具一定的的热稳定性和酸碱稳定性。Ｎ－乙酰－Ｄ氨基半乳能强烈地抑制ＢＶＬ对兔红细胞的凝集作用。乳糖、半乳糖也有较强的抑制定性。Ｎ
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| 关 键 词： | 凝集素 洋紫荆 亲和层析 纯化 理化性质 |
| 修稿时间： | 1999-04-01 |
Purificationand Characterization of Lectin from Bauhinia variegata L. |
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| PENG Jian-Zong CHEN Zhao-Ping CHENG Shuang-Qi. Purificationand Characterization of Lectin from Bauhinia variegata L.[J]. Chinese Bulletin of Botany, 2000, 17(3): 266-269 |
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| Authors: | PENG Jian-Zong CHEN Zhao-Ping CHENG Shuang-Qi |
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| Abstract: | Bauhinia variegata lectin(BVL) has been purified from the seed of Bauhinia variegata L. by affinity c hromatography on an acid-treated Sepharose 6B column . The hemagglutinating act ivity of the purified BVL increased 159 times and the activity recovery is 49.0 %. The molecular weight is 81 000 and the molecule consists of two identical sub units. Its isoelectric point is 4.95. N-acetyl-D-galactosamine is the most p otent inhibitors of BVL. Lactose and galactose can also inhibit the agglutinati on of rabbit erythocytes by BVL. |
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| Keywords: | Lectin Bauhinia variegata Aff inity chromatography Purification |
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