Co-administration of GP96 and Her2/neu DNA vaccine in a Her2 breast cancer model |
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Authors: | Nafiseh Pakravan Ladan Langroudi Monire Hajimoradi and Zuhair Mohammad Hassan |
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Institution: | (1) Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Ale-Ahmad Avenue, P.O. Box: 14115-331, Tehran, Iran |
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Abstract: | Heat-shock proteins have biochemical and immunological roles in chaperoning/signaling and activation of innate and adaptive
immune responses, respectively. Their effect on the immune response is due to a phenomenon known as cross-priming of antigen,
in which exogenous antigens are presented via MHC class I by antigen presenting cells. GP96 exerts adjuvant activity with
some viral and bacterial antigens when applied in the form of a DNA vaccine. In this study, animals with Her2-expressing tumors
were vaccinated by co-administration of GP96+ Her2/neu DNA vaccines. Analyses of the immune response, 2 weeks after the last
immunization revealed decreased CD4+ CD25+ Foxp3+ naturally occurring regulatory T cells (Tregs) at the tumor site and increased
IFN-γ/IL-4 level. Nevertheless, the graph of tumor size demonstrated a bi-phasic pattern in which partial control of tumor
progression initially occurred, but finally its effectiveness was inversely affected by tumor size. |
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Keywords: | Her2 GP96 Regulatory T cells Co-administration DNA vaccine |
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