PCR-mediated recombination and mutagenesis |
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Authors: | Robert M. Horton |
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Affiliation: | (1) Department of Biochemistry, College of Biological Sciences, 1479 Gortner Ave., 55108 St. Paul, MN |
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Abstract: | Gene Splicing by Overlap Extension (gene SOEing) is a sequence-independent method for site-directed mutagenesis and/or recombination of DNA molecules. It is based on the idea that a PCR product can be engineered by adding or changing sequences at its ends so that the product can itself be used to prime DNA synthesis in a subsequent overlap-extension reaction to create mutant or recombinant molecules. As the engineered genes are created in vitro without reliance on host organisms or restriction sites, gene SOEing provides a powerful and versatile tool for genetic investigation and engineering. |
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Keywords: | Polymerase chain reaction overlap extension recombinant PCR gene SOEing sitedirected mutagenesis |
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