Sterol composition of chitosomes from yeast cells of Mucor rouxii: Comparison with whole cells |
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| Authors: | Everardo Lopez-Romero Eduardo Monzon Jose Ruiz-Herrera |
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| Affiliation: | Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Mexico;Departamento de Genetica y Biologia Molecular, Centro de Investigacion y de Estudios Avanzados of the Instituto Politecnico Nacional, Mexico |
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| Abstract: | Abstract The free sterol composition of chitosomes and whole yeast cells of Mucor rouxii was determined by gas-liquid chromatography (GLC). The results obtained showed that, in contrast to whole cells, chitosomes are particularly rich in ergosterol: 87% vs. 29% of total free sterols. Three other sterols were present in varying amounts in both chitosomes and whole cells. These were identified by GLC as cholesterol, stigmasterol and sitosterol. These results indicate that the yeast cells preferentially accumulate ergosterol in chitosomes providing these microvesicles with a unique composition that may be important for the structure of the organelle. |
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| Keywords: | Mucor rouxii chitosome free sterols ergosterol |
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