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| 不对称还原苯乙酮的酵母菌株筛选与分子鉴定 | |
| 引用本文： | 朱冬眉,陈 丹,郑黎鹂,陈 华,杨志荣,孙 群.不对称还原苯乙酮的酵母菌株筛选与分子鉴定[J].微生物学通报,2008,35(12):1920-1925. |
| 作者姓名： | 朱冬眉 陈 丹 郑黎鹂 陈 华 杨志荣 孙 群 |
| 作者单位： | 1. 四川大学生命科学学院,成都,610064 2. 四川大学生命科学学院,成都,610064;四川省迈克科技有限责任公司,成都,611731 3. 四川大学化学学院,成都,610064 |
| 基金项目： | 四川省国际科技合作与交流研究计划项目"微生物资源的开发和利用"资助 |
| 摘 要： | 利用苯乙酮作为模式底物,对145株菌株进行初筛和复筛,获得一株具有高效立体选择性的酵母菌株YS6-2,能够不对称还原苯乙酮生成(S)-1-苯基乙醇.在苯乙酮浓度为70mmol/L时,底物的初始转化率达26.8%,产物(S)-1-苯基乙醇的对映体过量值为98.8%.基于形态学、生理生化特征、18S rDNA和26S rDNA D1/D2区域的分析表明,YS6-2为胶红酵母(Rhodotorula muci-laginosa). |
| 关 键 词： | 不对称还原 苯乙酮 酵母菌 系统发育 |
Screening and Molecular Identification of Yeast Strain for Asymmetric Reduction of Acetophenone |
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| ZHU Dong-Mei,CHEN Dan,ZHENG Li-Li,CHEN Hu,YANG Zhi-Rong and SUN Qun.Screening and Molecular Identification of Yeast Strain for Asymmetric Reduction of Acetophenone[J].Microbiology,2008,35(12):1920-1925. | |
| Authors: | ZHU Dong-Mei CHEN Dan ZHENG Li-Li CHEN Hu YANG Zhi-Rong and SUN Qun |
| Institution: | College of Life Sciences, Sichuan University, Chengdu 610064;(1. College of Life Sciences, Sichuan University, Chengdu 610064) (3. Sichuan Maker Science & Technology Co., Ltd, Chengdu 611731);College of Life Sciences, Sichuan University, Chengdu 610064;College of Chemistry, Sichuan University, Chengdu 610064;College of Life Sciences, Sichuan University, Chengdu 610064;College of Life Sciences, Sichuan University, Chengdu 610064 |
| Abstract: | Yeast strain YS6-2 with high stereoselectivity was screened from 145 strains when the asymmetric reduction of acetophenone to (S)-1-phenethylalcohol was chosen as the model reaction. With the concentration of acetophenone at 70 mmol/L, YS6-2 yielded (S)-alcohol at 26.8% conversion and enantiomeric excess reached up to 98.8%. Based on the analysis of 18S rDNA and 26S rDNA D1/D2 domain sequence, along with its general morphology, physiological and biochemical characteristics, YS6-2 was identified as Rhodotorula mucilaginosa. |
| Keywords: | 18S rDNA 26S rDNA D1/D2 |
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