Phenylalanine ammmonia-lyase activity in permeabilised yeast cells (Rhodotorula glutinis) |
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| Authors: | A. R. Srinivasan Nagajyothi L. R. Gowda S. G. Bhat |
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| Affiliation: | (1) Department of Biochemistry and Nutrition, Central Food Technological Research Institute, 570 013 Mysore, India |
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| Abstract: | Summary Whole cells of the yeast Rhodotorula glutinis IFO 0559 had low phenylalanine ammonia-lyase (PAL) activity due to a limited membrane permeability barrier for phenylalanine. Permeabilization with detergents and organic solvents increased cellular PAL activity significantly and of these cetyltrimethylammonium bromide gives the maximum increase (6-fold). PAL activity of such detergent permeabilized cells could be used for the synthesis and degradation of l-phenylalanine. |
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