Stimulation of embryogenesis and haploid production in Brassica campestris anther cultures by elevated temperature treatments |
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Authors: | W A Keller K C Armstrong |
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Institution: | (1) Ottawa Research Station, Research Branch, Agriculture Canada, Ottawa, Ontario, Canada |
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Abstract: | Summary Culture of Brassica campestris anthers at 35°C for one or three days prior to culture at 25°C significantly stimulated the yield of microspore-derived embryos. More than 100 plants were regenerated from cultured embryos and haploids were identified amongst them. The haploid frequency was greater than 70% if all small-flowered sterile plants were considered to be haploid. The yield of microspore-derived plants in B. campestris is approaching the level where anther culture may be utilized as a practical breeding tool. |
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Keywords: | Brassica campestris Embryogenesis Haploid Microspore Temperature |
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