Distribution and biodiversity of rhizobia nodulating Chamaecrista mimosoides in the Shandong peninsula of china |
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| Affiliation: | 1. Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin University, Yibin City, Sichuan Province 644000, China;2. College of Life Science, Yantai University, Yantai City, Shandong Province 264005, China;3. College of Resources and Environment, Shijiazhuang University, Shijiazhuang City, Hebei Province 050035, China;4. Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai City, Shandong Province 264003, China;5. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11340, Mexico;1. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China;2. Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, PR China;3. Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing 102206, PR China;4. Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu 476000, PR China;5. Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing 100191, PR China;6. Guangxi Key Laboratory of AIDS Prevention and Treatment & Guangxi Collaborative Innovation Center for Biomedicine, School of Public Health, Guangxi Medical University, Nanning 530021, Guangxi, PR China;7. Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, PR China;8. Research Institute of Public Health, Nankai University, Tianjin 300350, PR China;1. Department of Biology, University of Puerto Rico, Mayagüez, Puerto Rico;2. School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA, United States;3. School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, United States;4. Casa Pueblo, Adjuntas, Puerto Rico;1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People’s Republic of China;2. School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, People’s Republic of China;1. Department of Science, Roma Tre University, Viale G. Marconi 446, 00146 Rome, Italy;2. Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority (INAIL), Monte Porzio Catone, Rome, Italy;3. Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parco Area delle Scienze 11a, 43124 Parma, Italy;1. Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa;2. School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV, United States of America;3. MALDIID Pty Ltd, Murdoch, Western Australia, Australia;4. Laboratory of Microbiology, Department Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium;5. The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK;6. Universidade Estadual de Santa Cruz, km 16 Rodovia Ilhéus – Itabuna, CEP 45662-900 Ilhéus, BA, Brazil;7. Embrapa Cerrados, Cx. Postal 08223, 73310-970, Planaltina, Distrito Federal, Brazil;8. Biotechnology Platform, Agricultural Research Council Onderstepoort Veterinary Institute (ARC-OVI), Onderstepoort, South Africa |
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| Abstract: | Chamaecrista mimosoides is an annual herb legume widely distributed in tropical and subtropical Asia and Africa. It may have primitive and independently-evolved root nodule types but its rhizobia have not been systematically studied. Therefore, in order to learn the diversity and species affinity of its rhizobia, root nodules were sampled from C. mimosoides plants growing in seven geographical sites along the coast line of Shandong Peninsula, China. A total of 422 rhizobial isolates were obtained from nodules, and they were classified into 28 recA haplotypes. By using multilocus sequence analysis of the concatenated housekeeping genes dnaK, glnII, gyrB, recA and rpoB, the representative strains for these haplotypes were designated as eight defined and five candidate novel genospecies in the genus Bradyrhizobium. Bradyrhizobium elkanii and Bradyrhizobium ferriligni were predominant and universally distributed. The symbiotic genes nodC and nifH of the representative strains showed very similar topology in their phylogenetic trees indicating their co-evolution history. All the representative strains formed effective root nodules in nodulation tests. The correlation between genospecies and soil characteristics analyzed by CANOCO software indicated that available potassium (AK), organic carbon (OC) and available nitrogen (AN) in the soil samples were the main factors affecting the distribution of the symbionts involved in this current study. The study is the first systematic survey of Chamaecrista mimosoides-nodulating rhizobia, and it showed that Chamaecrista spp. were nodulated by bradyrhizobia in natural environments. In addition, the host spectrum of the corresponding rhizobial species was extended, and the study provided novel information on the biodiversity and biogeography of rhizobia. |
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| Keywords: | Nodule Coastal zone Rhizobia Diversity Distribution |
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