Paraburkholderia gardini sp. nov. and Paraburkholderia saeva sp. nov.: Novel aromatic compound degrading bacteria isolated from garden and forest soil samples |
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| Institution: | 1. Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia;2. Department of Biotechnology, TU Delft, the Netherlands;3. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Puschino, Moscow Region, Russia;4. Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Germany;1. Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China;2. China Ocean Sample Repository (Biology), Xiamen 361005, China;3. State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, China;1. Instituto Politécnico Nacional, Escuela Nacional de Ciencia Biológicas, Prolongación de Carpio y Plan de Ayala s/n, Col. Santo Tomás, Del. Miguel Hidalgo, 11340, México City, Mexico;2. Laboratorio de Ciencias AgroGenómicas, Escuela Nacional de Estudios Superiores, Universidad Nacional Autónoma de México, León, Guanajuato, Mexico;3. Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, 565-A, Cuernavaca, Morelos, Mexico;4. Department of Microbiology, University of Georgia, Athens, GA, 30602, United States;1. National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment of Shandong Agricultural University, Taian 271000, China;2. Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;3. College of Life Science, Yantai University, Yantai 264005, China;4. College of Marine Science, Shandong University at Weihai, Weihai 264209, China;5. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico;1. Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Marine Station Helgoland, Kurpromenade 201, 27498 Helgoland, Germany;2. GFZ German Research Centre for Geosciences, Section Geomicrobiology, Telegrafenberg, 14473 Potsdam, Germany;3. University of Göttingen, Institute of Microbiology and Genetics, Grisebachstr. 8, 37077 Göttingen, Germany;4. University of Potsdam, Institute of Biochemistry and Biology, 14469 Potsdam, Germany |
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| Abstract: | Three forest and four botanical garden top soil isolates with unique MALDI-TOF mass spectra were identified as Paraburkholderia strains closely related to Paraburkholderia sartisoli through recA gene sequence analysis. OrthoANIu, digital DNA-DNA hybridization analyses and phylogenomic analyses demonstrated that the five strains represented two new Paraburkholderia species closely related to P. sartisoli. The genome of strain LMG 31841T had a cumulative size of 6.3 Mb and a G + C content of 62.64 mol%; strain LMG 32171T had a genome size of 5.8 Mb and a G + C content of 62.91 mol%. Hemolysis on horse blood agar, beta-galactosidase and phosphoamidase activity, and assimilation of adipic acid and trisodium citrate allowed phenotypic differentiation of strains LMG 31841T, LMG 32171T and P. sartisoli LMG 24000T. An analysis of the genomic potential for aromatic compound degradation yielded additional differences among strains representing these three species, but also highlighted some discrepancies between the presence of genes and pathways, and the phenotype revealed through growth experiments using a mineral salts medium supplemented with single aromatic compounds as carbon sources. We propose to classify all isolates from the present study into two novel Paraburkholderia species, for which we propose the names Paraburkholderia gardini with LMG 32171T (=CECT 30344T) as the type strain, and Paraburkholderia saeva with LMG 31841T (=CECT 30338T) as the type strain. |
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| Keywords: | Paraburkholderia Novel species Microdiversity Aromatic compound degradation Comparative genomics |
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