Oxalate utilisation is widespread in the actinobacterial genus Kribbella |
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| Institution: | 1. College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, China;2. Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou, China;3. Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, China;1. Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia;2. Department of Biotechnology, Section of Environmental Biotechnology, TU Delft, The Netherlands;3. Institute of Polar Research, ISP-CNR, Messina, Italy;4. IAMC-CNR, Messina, Italy;5. NIOZ Royal Netherlands Institute for Sea Research, Department of Marine Microbiology and Biogeochemistry, and Utrecht University, Utrecht, The Netherlands;1. Ru?er Bo?kovi? Institute, Division of Materials Chemistry, Laboratory for Precipitation Processes, Bijeni?ka cesta 54, 10 000 Zagreb, Croatia;2. Croatian Geological Survey, Milan Sachs 2 Street, 10 000 Zagreb, Croatia;3. Joint Microbiome Facility of the Medical University of Vienna and the University of Vienna, Djerassiplatz 1, 1030 Vienna, Austria;4. Department of Laboratory Medicine, Medical University of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria;5. University of Vienna, Department of Microbiology and Ecosystem Science, Divison of Microbial Ecology, Djerassiplatz 1, 1030 Vienna, Austria;6. Center of Excellence for Science and Technology-Integration of Mediterranean Region (STIM), Split, Croatia;1. Winogradsky Institute of Microbiology, Research Centre of Biotechnology RAS, 7/2 Prospekt 60-letiya Oktyabrya, 117312 Moscow, Russia;2. Gubkin University, 65/1 Leninsky Prospect, 119991 Moscow, Russia;1. Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Mexico;2. School of Biosciences, University of Exeter, Exeter, UK;1. Department of Molecular Ecology, Hungarian University of Agriculture and Life Sciences, Gödöll?, Hungary;2. Department of Microbiology, Eötvös Loránd University, Budapest, Hungary;3. Institute of Horticultural Sciences, Laboratories of Food Analysis, Hungarian University of Agriculture and Life Sciences, Gödöll?, Hungary;4. Institute of Plant Biology, Biological Research Center, ELKH, Szeged, Hungary;5. Water Industry Engineering Department, Engineering Faculty, Kinneret Academic College on the Sea of Galilee, D.N. Emek Ha''Yarden, Israel;6. BioCastle Water Technologies Ltd., Edison Park, Israel;7. Department of Plant Anatomy, Eötvös Loránd University, Budapest, Hungary;8. Department of Environmental Safety, Hungarian University of Agriculture and Life Sciences, Gödöll?, Hungary |
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| Abstract: | The type strains of all 33 species in the genus Kribbella were tested for growth on oxalate (?OOC-COO?) as sole carbon source. Media were initially formulated to contain sodium oxalate, but even a concentration as low as 7.5 mM oxalate prevented growth. A modified medium based on calcium oxalate was very successful in characterising oxalate utilisation by Kribbella strains (metabolism of oxalate by oxalotrophic bacteria results in visible zones of clearing around the growth streaks on the opaque plates). To assess the variability of oxalate utilisation in Kribbella species, we also tested eight non-type strains for their ability to use oxalate. Thirty of 33 type strains (90.9%) and six of eight non-type strains (75%) were able to use oxalate as a sole carbon source. Based on these results, we propose that oxalate would be an excellent carbon source for the selective isolation of Kribbella strains. Based on the oxalate-utilisation phenotype and analyses of the 19 publicly available Kribbella type-strain genome sequences, we propose a pathway for oxalate metabolism in Kribbella. This pathway is significantly different from those previously proposed for oxalate metabolism in other bacteria, involving the indirect catabolism of oxalate to formate. Formate production is proposed to be involved in energy generation and to be crucial for oxalate import via an oxalate:formate antiporter. To our knowledge, this is the first report of an oxalate:formate antiporter in an aerobic, Gram-positive bacterium. |
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| Keywords: | Oxalic acid Oxalate Oxalotrophy Selective isolation Oxalate:formate antiporter |
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