Neomycin: An Effective Inhibitor of Jasmonate-Induced Reactions in Plants |
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Authors: | Vadassery Jyothilakshmi Ballhorn Daniel J. Fleming Steven R. Mazars Christian Pandey Shree P. Schmidt Axel Schuman Meredith C. Yeh Kai-Wun Yilamujiang Ayufu Mithöfer Axel |
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Affiliation: | 1.Department of Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, Hans-Knöll-Str. 8, 07745, Jena, Germany ;2.National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, New Delhi, 110067, India ;3.Department of Biology, Portland State University, Portland, OR, 97207-0751, USA ;4.Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745, Jena, Germany ;5.Centre National de la Recherche Scientifique, UMR 5546, 31326, Castanet-Tolosan, France ;6.Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia, 741246, India ;7.Department for Molecular Ecology, Max Planck Institute for Chemical Ecology, 07745, Jena, Germany ;8.Institute of Plant Biology, National Taiwan University, Taipei, 106, Taiwan, ROC ; |
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Abstract: | Jasmonates are important phytohormones involved in both plant developmental processes as well as defense reactions. Many JA-mediated plant defense responses have been studied in model plants using mutants of the jasmonate signaling pathway. However, in plant species where JA-signaling mutants are not accessible, the availability of a tool targeting JA signaling is crucial to investigate jasmonate-dependent processes. Neomycin is a poly-cationic aminoglycoside antibiotic that blocks the release of Ca2+ from internal stores. We examined the inhibitory activities of neomycin on different jasmonate-inducible responses in eight different plant species: Intracellular calcium measurements in Nicotiana tabacum cell culture, Sporamin gene induction in Ipomoea batatas, PDF2.2 gene expression in Triticum aestivum, Nepenthesin protease activity measurement in Nepenthes alata, extrafloral nectar production in Phaseolus lunatus, nectary formation in Populus trichocarpa, terpene accumulation in Picea abies, and secondary metabolite generation in Nicotiana attenuata. We are able to show that neomycin, an easily manageable and commercially available compound, inhibits JA-mediated responses across the plant kingdom. |
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