Morphological and genomic characteristics of two novel halotolerant actinomycetes,Tomitella gaofuii sp. nov. and Tomitella fengzijianii sp. nov. isolated from bat faeces |
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| Affiliation: | 1. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China;2. Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, PR China;3. Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing 102206, PR China;4. Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu 476000, PR China;5. Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing 100191, PR China;6. Guangxi Key Laboratory of AIDS Prevention and Treatment & Guangxi Collaborative Innovation Center for Biomedicine, School of Public Health, Guangxi Medical University, Nanning 530021, Guangxi, PR China;7. Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, PR China;8. Research Institute of Public Health, Nankai University, Tianjin 300350, PR China;1. Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin University, Yibin City, Sichuan Province 644000, China;2. College of Life Science, Yantai University, Yantai City, Shandong Province 264005, China;3. College of Resources and Environment, Shijiazhuang University, Shijiazhuang City, Hebei Province 050035, China;4. Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai City, Shandong Province 264003, China;5. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11340, Mexico;1. IFAPA Centro Las Torres, Crta Sevilla-Cazalla Km 12, 2, 41200 Seville, Spain;2. Dpto. Microbiología y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, Granada, Spain;1. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan Province 450000, PR China;2. Collaborative Innovation Center for Food Production and Safety of Henan Province, Zhengzhou 450002, Henan Province, PR China;3. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China;4. LSTM, Univ Montpellier, CIRAD, INRAE, Institut Agro, IRD, Montpellier, France;5. Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand;6. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 México, D. F, Mexico;1. iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal;2. Departamento de Microbiologia, Laboratório de Microbiologia e Bioprocessos, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, Brazil;1. Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia;2. Department of Biotechnology, TU Delft, the Netherlands;3. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Puschino, Moscow Region, Russia;4. Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Germany;1. Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia;2. Department of Biotechnology, Section of Environmental Biotechnology, TU Delft, The Netherlands;3. IAMC-CNR, Spianata S.Raineri 86, 98122 Messina, Italy;4. NIOZ Royal Netherlands Institute for Sea Research, Department of Marine Microbiology and Biogeochemistry, and Utrecht University, PO Box 59, 1790 AB Den Burg, Texel, The Netherlands;5. Faculty of Geosciences, Department of Earth Sciences, Utrecht University, Utrecht, The Netherlands |
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| Abstract: | Four white-pigmented, Gram-staining-positive, strictly aerobic, non-spore-forming, irregular rod-shaped bacteria were isolated from the faeces of bats collected from Guangxi autonomous region (22°20′54″N, 106°49′20″E; July 28, 2011) and Chongqing city (30°02′15″N, 107°07′4″E; September 1, 2011) of South China. The strains shared 99.3–99.9% 16S rRNA gene sequence similarity by BLAST search among them, and belonged to genus Tomitella according to 16S rRNA gene and genomic sequence-based phylogenetic/phylogenomic analyses. Strains HY172T and HY188T contained meso-diaminopimelic acid as the diagnostic diamino acid, and arabinose, glucose, galactose or ribose in their whole-cell hydrolysates. Besides sharing phosphatidylethanolamine, diphosphatidylglycerol and unidentified glycolipid(s) in their polar lipid profiles, additionally HY172T had one unidentified phosphoglycolipid and three unidentified phospholipids whereas HY188T had phosphatidyl inositol mannoside and four unidentified aminolipids. The main cellular fatty acids of strains HY172T and HY188T were C16:0, C18:0 10-methyl, C18:1ω9c and summed feature 3. The genomic DNA G + C contents of both strains (HY172T and HY188T) were 70.9 %. The genus Tomitella contains 2311 core genes, and resuscitation promoting factor (rpf) genes can be found in all members of Tomitella. The digital DNA-DNA hybridization and average nucleotide identity values of the four novel strains with other members of the genus Tomitella were within the ranges of 20.1–45.2% and 74.8–91.9%, respectively, all below the respective recommended 70.0% and 95–96% cutoff point. Based on phylogenetic, chemotaxonomic and phenotypic analyses, these four strains could be classified as two novel species of the genus Tomitella, for which the names Tomitella gaofuii sp. nov. and Tomitella fengzijianii sp. nov. are proposed. The type strains are HY172T (= CGMCC 1.18701T = JCM 34231T) and HY188T (= CGMCC 1.16971T = JCM 33467T), respectively. |
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| Keywords: | Bat Resuscitation promoting factor |
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