Rhizobium cremeum sp. nov., isolated from sewage and capable of acquisition of heavy metal and aromatic compounds resistance genes |
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| Affiliation: | 1. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan Province 450000, PR China;2. Collaborative Innovation Center for Food Production and Safety of Henan Province, Zhengzhou 450002, Henan Province, PR China;3. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China;4. LSTM, Univ Montpellier, CIRAD, INRAE, Institut Agro, IRD, Montpellier, France;5. Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand;6. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 México, D. F, Mexico;1. Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia;2. Department of Biotechnology, Section of Environmental Biotechnology, TU Delft, The Netherlands;3. IAMC-CNR, Spianata S.Raineri 86, 98122 Messina, Italy;4. NIOZ Royal Netherlands Institute for Sea Research, Department of Marine Microbiology and Biogeochemistry, and Utrecht University, PO Box 59, 1790 AB Den Burg, Texel, The Netherlands;5. Faculty of Geosciences, Department of Earth Sciences, Utrecht University, Utrecht, The Netherlands;1. Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technology Applications (SRTA-City), Alexandria, Egypt;2. Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain;3. Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), Salamanca, Spain;4. Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA-CSIC, Spain;5. BioTechnology Institute and Department of Soil, Water & Climate, University of Minnesota, St. Paul, USA;1. Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy Prospect, 33, bld. 2, 119071 Moscow, Russia;2. Gubkin University, Leninskiy Prospect, 65/1, 119991 Moscow, Russia;1. Centro de Biotecnología y Genómica de Plantas (CBGP), Universidad Politécnica de Madrid – Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28223 Pozuelo de Alarcón, Madrid, Spain;2. Departamento de Biotecnología y Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, 28040 Madrid, Spain;3. Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), Salamanca, Spain;4. Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA (CSIC);5. Instituto de Ciencias Agrarias, Consejo Superior de Investigaciones Científicas (ICA-CSIC), 28006 Madrid, Spain;1. Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia;2. Department of Biotechnology, TU Delft, the Netherlands;3. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Puschino, Moscow Region, Russia;4. Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Germany |
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| Abstract: | Two strains of Rhizobia isolated from sewage collected from the Chinese Baijiu distillery were characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains W15T and W16 were grouped as a separate clade closely related to Rhizobium daejeonense L61T (98.6%). Multilocus sequence analysis (MLSA) with three housekeeping genes (recA, glnII and rpoA) also showed that strains W15T and W16 belonged to the genus Rhizobium. Average nucleotide identity and digital DNA–DNA hybridization values between genome sequences of strain W15T and the closely related species ranged from 77.0% to 87.8% and from 23.9% to 30.9%. The DNA G + C content of strain W15T was 61.6 mol%. Strain W15T contained Q-10 as the major ubiquinone and the dominant fatty acids were summed feature 8 (C 18:1ω7c and/or C 18:1ω6c; 73.1%) and C18:0 (7.6%). The main polar lipids are phosphatidylcholine, phosphatidylmethylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. On the basis of the evidences presented in this study, strains W15T and W16 represents a novel species of the genus Rhizobium, for which the name Rhizobium cremeum sp. nov. is proposed. The type strain is W15T (= CGMCC 1.18731T = KACC 22344T). |
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| Keywords: | New taxa Taxonomy |
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